EN | TR
E-ISSN: 3061-9947
Priority of Alpha-L- Fucosidase Over Alpha fetoprotein in Early Detection of Hepatocellular Carcinoma in Animals and Human
PDF
Cite
Share
Request
Original Article
VOLUME: 47 ISSUE: 2
P: 1-7
2014

Priority of Alpha-L- Fucosidase Over Alpha fetoprotein in Early Detection of Hepatocellular Carcinoma in Animals and Human

Acta Haematol Oncol Turc 2014;47(2):1-7
DOI: 10.5505/aot.2014.02886
Nabil Mohie Abdel Hamid
Maiada Hasan Nazmy
Bakheet Elkot Moustafa
Sahar Mohamed Saad El-din
1. Department Of Biochemistry, Faculty Of Pharmacy, Kafrelsheikh University, Kafrelsheikh, Egypt.
2. 2department Of Biochemistry, Faculty Of Pharmacy, Minia University, Minia, Egypt.
3. Department Of Biochemistry, Faculty Of Pharmacy, Al-azhar University, Assiut, Egypt.
4.
No information available.
No information available
Received Date: 2014-08-26T19:45:06
Accepted Date: 2014-10-30T14:38:25
PDF
Cite
Share
Request

Abstract

OBJECTIVE

Early detection of hepatocellular carcinoma (HCC) is the most critical step in the management process. We try to provide new insights about the possible role of alpha-L- fucosidase (AFU) to achieve more reliable detection of HCC over a classical marker alpha fetoprotein (AFP).

METHODS

The study included both animal and human subjects, to legislate our theory. In animal study, 60 Male Wistar rats were classified to control, other 3 groups received di ethyl nitrosamine (DENA) for 8, 16, 24 weeks respectively. In human study: one hundred and twenty patients were assigned as: control group, 3 groups with histologically graded HCC, into mild, advanced and metastatic HCC. Histological and biochemical analysis (AFP, AFU, liver function tests, total antioxidant serum levels) in both animals and patients, as well as, receiver operating characteristic (ROC) curves and correlation analysis, were executed.

RESULTS

DENA-treated groups showed significant histopathological changes in a time dependant pattern. After 24 weeks, a significant variation in biochemical parameters (AFP,AFU, liver function tests and total anti-oxidant serum levels), after 6 weeks, a significant variations in all parameters except bilirubin and total antioxidants, after 8 weeks, a significant variations in all parameters except bilirubin, albumin, AFP and total antioxidants, compared to control group. All tested biochemical parameters showed non-significant correlation, compared to AFU. All histologically-graded human HCC groups showed a highly statistically significant variations in all tested biochemical parameters. ROC analysis showed that at a cut-off value of 5 μmol/l/min, AFU yielded a sensitivity and specificity of 90% and 92%, respectively producing diagnostic accuracy of 91%. While At a cut-off value of 60 ng/ml, AFP yielded a sensitivity and specificity of 60% and 76%, respectively producing diagnostic accuracy of 68%. Correlation coefficients between AFU versus ALT, AST and AFP were statistically significant

CONCLUSION

AFU is a more accurate marker than AFP for early diagnosis of HCC. AFU showed higher sensitivity, specificity and correlated more to other indices than AFP. Human data confirmed our theory.