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E-ISSN: 3061-9947
Cytotoxic Effects Of N-(4-Chlorophenyl)-1H-Indole-2-Carboxamide On Bone Cancer Cells
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Original Article
VOLUME: 53 ISSUE: 1
P: 173-180
2020

Cytotoxic Effects Of N-(4-Chlorophenyl)-1H-Indole-2-Carboxamide On Bone Cancer Cells

Acta Haematol Oncol Turc 2020;53(1):173-180
DOI: 10.5505/aot.2020.20591
Medine Taşdemir 1
Mete Emir Özgürses 1
Hakan Darıcı 2
Nazlı Ece Ordueri 3
1. Biruni University, Faculty of Engineering and Natural Sciences Faculty, Department of Molecular Biology and Genetics, Istanbul, Turkey
2. Istinye University, Faculty of Medicine, Department of Histology and Embryology, Istanbul, Turkey
3. Biruni University, Faculty of Medicine, Department of Histology and Embryology, Istanbul, Turkey
4.
No information available.
No information available
Received Date: 2019-08-14T10:59:34
Accepted Date: 2020-05-14T14:45:08
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Abstract

INTRODUCTION

Osteosarcoma is a common tumor of high malignancy, usually derived from mesenchymal tissues. The lack of response to chemotherapeutic treatments indicates the importance of investigating new therapeutic methods. Indole, which is known to inhibit proliferation on cancer cells, is new synthesized as a derivative of this molecule of N- (4-chlorophenyl) -lH-indole-2-carboxamide, has an effect on osteosarcoma cell line. We evaluated the cytotoxic effects of N- (4-chlorophenyl) -1H-indole-2-carboxamide by verification of pharmacological doses of imatinib mesylate based on time on osteosarcoma cells.

METHODS

: In the present study, the effects of N-(4-chlorophenyl)-1H-indole-2-carboxamide and Imatinib mesylate doses on cell viability of osteosarcoma Saos-2 cells were conducted using MTT assay. Ezrin expression levels was examined by immunofluorescence staining.

RESULTS

The results of the antiproliferative activity showed that N- (4-chlorophenyl) -1 H-indole-2-carboxamide inhibited cell proliferation of Saos-2 cells in a dose- and time-dependent manner. We found that even lower doses of N- (4-chlorophenyl) -1H-indole-2-carboxamide was effective on osteosarcoma cell lines when we compared with imatinib mesylate pharmacological doses.

DISCUSSION AND CONCLUSION

In this study, for the first time the newly synthesized indole derivative molecule, N- (4-chlorophenyl) -1 H-indole-2-carboxamide was evaluated for resistance in vitro Saos-2 cell line. All these results showed that N- (4-Chlorophenyl) -1 H-Indole-2-Carboxamide can be developed as a potential therapeutic agent for inhibiting osteosarcoma growth and metastasis