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Bax Gene Polymorphism in Childhood Acute Lymphoblastic Leukemia and Evaluation of Apoptosis with Conventional Dose-Prednisolone Treatment
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Original Article
VOLUME: 52 ISSUE: 3
P: 356-363
2019

Bax Gene Polymorphism in Childhood Acute Lymphoblastic Leukemia and Evaluation of Apoptosis with Conventional Dose-Prednisolone Treatment

Acta Haematol Oncol Turc 2019;52(3):356-363
DOI: 10.5505/aot.2019.29981
Elif Güler Kazancı 1
Ferah Genel 2
Hüseyin Onay 3
Ferda Özkinay 3
Canan Vergin 4
1. High Specialization Training and Research Hospital, Pediatric Hematology and Oncology, Bursa
2. Dr. Behçet Uz Pediatric Diseases and Surgery Training and Research Hospital Pediatric Immunology, İzmir
3. Ege University Faculty of Medicine Medical Genetics Department, İzmir
4. Dr. Behçet Uz Pediatric Diseases and Surgery Training and Research Hospital Pediatric Hematology and Oncology, İzmir
5.
No information available.
No information available
Received Date: 2019-05-15T14:37:21
Accepted Date: 2019-12-27T14:18:42
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Abstract

INTRODUCTION

It is known that the agents used in cancer treatment increase apoptosis and cause cell death. In this study, the importance of G-248A nucleotide polymorphism affecting the BAX gene expression and apoptosis in children with acute lymphoblastic leukemia (ALL) was investigated in the risk classification and the evaluation of the treatment response.

METHODS

Apoptosis index was performed by Annexin V- FITC kit for bone marrow aspiration samples of 25 newly-diagnosed (0-11) years old ALL patients on 0th, 3rd and 8th day. Bax gene polymorphism was studied on blood samples of 60 ALL patients and 96 healthy control group, and DNA isolation was studied by FUJİ QuickGene 810 automatic nucleic acid purification system.

RESULTS

The mean age of the patients was 4.9, and %28 had a standard risk, %44 had a moderate risk, %28 had high risk. A significant difference and a positive correlation were found between the spontaneous apoptosis index (AI) and the 3rd day AI (18.3±13.7, 19.4±12.7 p= 0,007 r= +0.548). The 8th day AI was found to be significantly higher in patients with poor early steroid response (p<0.05). Apoptosis index; was significantly high on 0th, 3rd and 8th day for PreB ALL cases. 84% of the patients were found to have CD 10 positivity and in cases with intense CD10 expression, AI was significantly higher on the third day (p <0.05). It was determined that the BAX gene polymorphism did not differ in the patient and control groups.

DISCUSSION AND CONCLUSION

In the present study, conventional dose prednisolone was thought to induce apoptosis significantly, and the 8th day AI elevation was associated with blast load in patients with poor early steroid response.It was concluded that the significant increase in apoptosis ratio in cases with intense CD10 expression was a valuable marker in terms of prognosis in Pre B ALL.It was considered necessary to evaluate the effect of BAX gene polymorphism on the pathogenesis, clinical course and prognosis of ALL with more cases, and to investigate other proteins that play a role in the apoptosis pathway.