The Effects of Concomitant Giardia intestinalis Infection on Acute Gastrointestinal Toxicity in Rats Undergoing Pelvic Irradiation

INTRODUCTION: Different types of pelvic cancer, such as cervical, endometrial, bladder and prostate, are normally treated by radical radiotherapy, which can be used both alone or in combination with surgery and/or chemotherapy.The aim of this study is to assess the effects of concomitant Giardia intestinalis infection on acute gastrointestinal toxicity in rats that have undergone pelvic irradiation.
METHODS: The study group consisted of forty female 6-month-old Wistar rats with the weight of 250 g. The rats were divided into four groups containing ten rats in each group. The study groups are as follows: Group 1 contained rats not infected with Giardia intestinalis and not irradiated, Group 2 contained rats infected with Giardia intestinalis but not irradiated, Group 3 contained rats not infected with Giardia intestinalis but irradiated, Group 4 contained rats infected with Giardia intestinalis and radiated. For the day after the end of radiation, the number of stool pellets was counted, and the operation of weighing rats was performed, and they were sacrificed the following day. The intestinal tissues were taken for histological evaluation.
RESULTS: A mucosal damage, such as villus shortening, atrophy of surface epithelium, crypt loss, as well as a decrease in the number of goblet cells of the group 3 and 4, was detected as a result of the light microscopic examination.
DISCUSSION AND CONCLUSION: As a result of the present study, the fact that concomitant Giardia intestinalis infection aggravates acute gastrointestinal toxicity in rats that have undergone pelvic irradiation has been verified

Pelvik Radyoterapi Uygulanýlan Sýçanlarda Akut Gastointestinal Toksisite Üzerine Giardi Ýntestinalis Enfeksiyonunun Etkileri

GÝRÝÞ ve AMAÇ: Servikal, endometrial, mesane ve prostat gibi pelvik kanserin farklý türleri normalde ya sadece radikal radyoterapi ile yada cerrahi ve kemoterapi birlikte kombinasyon halinde tedavi edilebilir. Bu çalýþmanýn amacý, pelvik radyasyona maruz kalan sýçanlarda eþzamanlý Giardia intestinalis enfeksiyonunun akut gastrointestinal toksisite üzerine etkilerinin araþtýrýlmasýdýr.
YÖNTEM ve GEREÇLER: Çalýþma grubu, 250 g aðýrlýða sahip kýrk adet 6 aylýk diþi Wistar sýçandan oluþmaktadýr. Sýçanlar, herbir grupta 10 adet sýçan olacak þekilde dört gruba ayrýldý. Çalýþma gruplarý; Grup 1, Giardia intestinalis ile enfekte olmayan ve radyoterapi almayan sýçanlar, Grup 2, radyoterapi almamýþ, ancak Giardia intestinalis ile enfekte olan sýçanlar, Grup 3, Giardia intestinalis ile enfekte olmamýþ fakat radyoterapi almýþ sýçanlar, Grup 4'te hem Giardia intestinalis ile enfekte olan hemde radyoterapi alan sýçanlardan oluþmaktadýr. Radyasyon bittikten sonraki gün, hayvan vücut aðýrlýklarý kayýtedildi ve dýþkýlama sýklýðý hesaplandý. Ratlar perfore edilerek sakrifiye edildi, ince baðýrsak dokularý histolojik inceleme için alýndý.
BULGULAR: Iþýk mikroskopik incelemesinin sonucu olarak, grup 3 ve 4 ‘te villus kýsalmasý, yüzeyel epitelinde atrofi, kriptalarda kayýp gibi mukozal hasarlar ve goblet sayýsýnda azalma tespit edildi.
TARTIÞMA ve SONUÇ: Bu çalýþmanýn bir sonucu olarak, Giardia intestinalis enfeksiyonu ile eþzamanlý olarak pelvik radyoterapi uygulanmasý sýçanlarda akut gastrointestinal toksisiteyi arttýrmýþtýr.